An+experiment+of+HeLa

According to a 10-day cell culture experiment involving HeLa cells shown by the University of Maryland (2011), [3] the experiment was observed that the cell growth in the culture suspension doubled after 23hours, with maximum cell densities of approximately 3 - 4.105cells/ml. Averages of 70.106 HeLa cells were harvested daily from day 5 of the HeLa cells culture. [2]
 * Characteristic Growth of HeLa Cells:**

Figure1: A graphical representation of growth rates of HeLa cells in cells suspension and Cultispher from the University of Maryland (2011). [3]

The HeLa cell growth trends were observed in both the culture suspension and CultiSpher-G. [3] However, Meck, Carsten, and Kelsch (1976) [2] observe that human tumour cells grow more rapidly in diffusion chamber cultures. The diffusion chamber cultures are more advantageous since they allow assaying of metabolites from chemotherapeutic drugs' effects and the host-mediated metabolites. [2] Moreover, diffusion chamber cultures makes it possible to measure acellular products from the cultured tumour cell such as antigens. [1] Cell evasion of senescence apoptotic death and subsequent immortalization forms the basis for tumorigenesis and cancer biology. [1]

By YongMin KIM //Last edited 11/11/2011//


 * References:**

[1] Chung, D. C. (2010). //The Massachusetts General Hospital Guide to Clinical Cancer Genetics.// Boston, USA: Springer. pp. 15-17.

[2] Meck, R. A. L., & Kelsch, J. J. (1976). Growth of HeLa Cells in Diffusion Chamber cultures in Vivo. //Cancer Research//, Vol. 36: 2317-2320.

[3] University of Maryland, (2011). //Impact of HeLa.// //Available: []// __/ Resources/5.pdf __. [2011. 5/11/2011]